Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGQSFNAPYEAIG-EELLSQLVDTFYERVASHPLLKPIFP-SDLTETARKQKQFLTQYLGGPPLYTEEHGHPMLRARHLPFPITNERADAWLSCMKDA---MDHVGLEGEIREFLFGRLELTARHMVNQTEAEDRSS 1NGK Chain:H ((2-125)) -PKSF---YDAVGGAKTFDAIVSRFYAQVAEDEVLRRVYPEDDLAGAEERLRMFLEQYWGGPRTYSEQRGHPRLRMRHAPFRISLIERDAWLRCMHTAVASIDSETLDDEHRRELLDYLEMAAHSLVN---------

General information: TITO was launched using: RESULT: Template: 1NGK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 393 -57931 -147.41 -486.81 target 2D structure prediction score : 0.88 Monomeric hydrophicity matching model chain H : 0.79 3D Compatibility (PKB) : -147.41 2D Compatibility (Sec. Struct. Predict.) : 0.88 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.630

(partial model without unconserved sides chains): PDB file : Tito_1NGK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NGK-query.scw PDB file : Tito_Scwrl_1NGK.pdb: