Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTIKTSNLGFPRIGLNREWKKALEAYWKGSTDKDTFLKQIDELFLSAVKTQIDQQIDVVPVSDFTQYDHVLDTAVSFNWIPKRFRHLTD-ATDTYFAIARGIK----DAVSSEMTKWFNTNYHYIVPEYDESIEFRLTR-NKQLEDYRRIKQEYGVETKPVIVGPYTFVTLAKGYEP--SEAKAIQKRLVPLYVQLLKELEEEGVKWVQIDEPALVTASSED-VRGAKELFESITSEL-SSLNVLLQTYFDSVDAYEELIS-Y-PVQGIGLDFVHDKGRNLEQLKTHGFPTDKVLAAGVIDGRNIWKADLEERLDAVLDILSIAKVDELWIQPSSSLLHVPVAKHPDEHLEKDLLNGLSYAKEKLAELTALKEGLVSGKAAISEEIQQAKADIQALKQFATGANSEQKKELEQLTDKDFKRPIPFEERLALQNESLGLPLLPTTTIGSFPQSAEVRSARQKWRKAEWSDEQYQNFINAETKRWIDIQEELELDVLVHGEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIYGDVEFIEPMTVKDTVYAQSLTSKHVKGMLTGPVTILNWSFPRNDISRKEIAFQIGLALRKEVKALEDAGIQIIQVDEPALREGLPLKTR-DWDEYLTWAAEAFRLTTSSVKNETQIHTHMCYSNFEDIVDTINDLDADVITIEHSRSHGGFLDYLKNHPYLKGLGLGVYDIHSPRVPSTEEMYNIIVDALAVCPTDRFWVNPDCGLKTRQQEETVAALKNMVEAAKQARAQQTQLV 4ZTY Chain:A ((2-766)) ---VQSSVLGFPRMGVLRDLKKANEAYWADKISQEALLAEGKRLRLAHWKIQKDAGVDIIPSNDFAHYDHVLDHIQLFNAVPERYTSQKLSPLDEYFAMGRGHQKGGVDVPALEMVKWFDSNYHYVKPTLQDNQTFSLAKDPKPVREFLEAK-EAGFQTRPVLVGPVSFLALGKADRGSSVDPITLLDKLVPVYVELLKQLKAAGAESVQIDEPVLVFDLRPEVKAAFKPAYEAIAAAGDAVPKVVVATYFGDIVHNFDVLPAFSGAAGLHVDLVRNP-EQLEPVLK-QLGPNQILSAGVVDGRNIWKNDFAKSLEILQTAVKALGSERVIVATSSSLIHTPHTLASEKKLPSDVYEWFSFAVEKVKEVATLAKAVTEPEA-VKAELEANAAAIKARTDSKRTNDPAVKERQAQVTPEQHNRKAPFNTRYAEQKKHLSLPLFPTTTIGSFPQTSEIRVQRNKFTKGEISAEEYERFIEKEIELAVKIQDELDLDVYVHGEPERNDMVQYFGERLNGYVFTTHAWVQSYGSRCVRPPIIVGDISRPAPMTVKESKYAASISKKPMKGMLTGPVTCLRWSFPRVDVHQSVQCQQLALALRDEVVDLEKNGIYVIQVDEPALREGLPLRKGQEREAYLKWAVDSFKLATAGVENSTQIHSHFCYSEFQDFFHAIAALDADVLSIENSKSDAKLLKVFIDEEYPRHIGPGVYDIHSPRVPTLEEFKQRIEEMLAYLKPEQLWINPDCGLKTRKWDEVKGALSHMVEAAKYFREKYA---

General information: TITO was launched using: RESULT: Template: 4ZTY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4384 -316106 -72.10 -420.35 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -72.10 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_4ZTY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZTY-query.scw PDB file : Tito_Scwrl_4ZTY.pdb: