Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQMRFTEEDFNTFTIEGLDARMEVLKETVRPKLTALGEHFAPTLSALTGDEMF-PHVAKHARRSVNPP-ADSWVAFANSKRGYKKLPHFQIGLWESHVF-VWFAIIYE-SPIKEEYGKLLEVNQETITKNIPDSFVWSADHTKPGVHKQSEMDKEQLKTLFERLQTVKKAELLCGIQLQKEEVLNMNNQEFLQRIDDAFKQLA-F-LYRLTQKVTQA 3CZ6 Chain:A ((4-154)) -RSDFSNEDIYDNIDPDTISF----------PPKIATTDLFLPLFFHFGSTRQFMDKLHEVISGDYEPSQAEKLVQDLCDETGIRKNFSTSILTCLSGDLMVFPRYFLNMFK-----------DNVNPPPNVPG--IWT---------------------------------------HDDDESLKSNDQEQIRKLVKKHGTGRMEMRKRFFEK----

General information: TITO was launched using: RESULT: Template: 3CZ6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 515 -42625 -82.77 -293.96 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -82.77 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.312

(partial model without unconserved sides chains): PDB file : Tito_3CZ6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CZ6-query.scw PDB file : Tito_Scwrl_3CZ6.pdb: