Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTINIVAGGPKNLIPDLTGYTDEHTLWIGVDKGTVTLLDAGIIPVEAFGDFDSITEQERRRIEKAAPALHVYQAEKDQTDLDLALDWALEKQPDIIQIFGITGGRADHFLGNIQLLYKGVK--TNIKIRLIDKQNHIQMFPPGEYDIEKDENKRYISFIPFSEDIHELTLTGFKYPLNNCHITLGSTLCISNELIHSRGTFSFAKGILIMIRSTD 3L8M Chain:A ((1-211)) -MKANLLCGNRNLPKHILV--EHKHEHWIGIDRGTLILLESGITPQFAVGDFDSISDSERNFIQQ-QIEINPYN---DDTDLALGIDQAVKRGYRNIDVYGATGGRLDHFMGALQILEKPEYAKMNINIKLIDDTNEIQFIQKGQFNV-----FPYISFIPVI-P-TVISLKGFKYNLQNE-------LTISNEL---CGNIEIIEGSVLMIRSKD

General information: TITO was launched using: RESULT: Template: 3L8M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 961 -103215 -107.40 -543.24 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -107.40 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_3L8M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L8M-query.scw PDB file : Tito_Scwrl_3L8M.pdb: