Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVQIIFDSKTGNVQRFVNKTG--FQQI--R-K-----VD---------EMDHVDTPFVLVTYTTN-FG-------QVPASTQSFLE--KYAHLLLGVAASGNKVWGDNFAKSADTISRQYQVPILHKFELSGTSKDVELFTQEVERVVTKSSAKMDPVK 4N82 Chain:B ((23-174)) KVSLVYISLSGNTESFVRRLTDYLLEQHPSLEVEKIHIKDLVKERQPFFEMDNPFIAFLPTYLEGGNGVDNGDVEILTTDVGDFIAYGQNASKCLGVIGSGNRNFNNQYCLTAKQYSERFGFPVLADFEMRGMLGDIKKVAGIIEELYHIEK-------

General information: TITO was launched using: RESULT: Template: 4N82.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 562 -75563 -134.45 -614.33 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -134.45 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_4N82.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N82-query.scw PDB file : Tito_Scwrl_4N82.pdb: