Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANEQKTAAKDVFQARKTFTTNGKTYHYYSLK-ALEDSGIGKVSKLPYSIKVLLESVLRQVDGFVIKKEHVENLAKWGTAELKDI-DVPFKPSRVILQDFTGVPAVVDLASLRKAMAAVGGDPDKINPEIPVDLVIDHSVQVDKAGTEDALAVNMDLEFERNAERYKFLS-WAKKAFNNYQAVPPATGIVHQVNLEFLASVVHAIEEDGELVTYP-DTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPVPEVIGAKLVGKLPNGTTATDLALKVTQVLREKGVVGKFVEFFGPGIAELPLADRATIANMAPEYGATCGFFPVDEEALNYLRLTGRDPEHIDVVEAYCRSNGLFYTPDAEDPQFTDVVEIDLSQIEANLSGPKRPQDLIPLSAMQETFKKQLVSPAGNQGFGLNAEEENKEIKFKLLNGEETVMKTGAIAIAAITSCTNTSNPYVLIGAGLVAKKAVELGLKVPNYVKTSLAPGSKVVTGYLVNSGLLPYMKELGFNLVGYGCTTCIGNSGP--L-SPEIEEAVAKNDLLITSVLSGNRNFEGRIHP-LVKGNYLASPPLVVAYALAGTVNINLKTDPIGVGKDGQNVYFNDIWPSMDEINALVKQTVTPELFRKEYETVFDDNKRWNEIETT-DEALYKWDNDSTYIQNPPFFEEMSVEPGKVEPLKGLRVVGKFGDSVTTDHISPAGAIGKDTPAGKYLQEKGVSPRDFNSYGSRRGNHEVMMR-GTFANIRIKNQIAPGTEGGFTTYWPTGEVTSIYDACMKYKEDKTGLVVLAGKDYGMGSSRDWAAKGTNLLGIRTVIAESFERIHRSNLVFMGVLPLQFKQGENADTLGLTGKEVIEVDV-DETVRPRDLVTVRAINEDGNVTTFEAVVRFDSEVEIDYYRHGGILQMVLREKMKQS 1C96 Chain:A ((13-752)) -----------------------EYIRYDLLEKNIDIVRKRLNRPLTLSEKIVYGHLDDPAN------QEIE----------RGKTYLRLRPDRVAMQDATAQMAMLQFISS-------GL-PKVA---VPSTIHCDHLIEAQLGG-----EKDLRRAKDINQEVYNFLATAGA--KYGVGFWRPGSGIIHQIILENY--------------AYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADI-ANLADEFK--DHLVPDP---GCHYDQVIEINLSELKPHINGPFTPDLAHPVAEVGSVAE--------------------------------KEGWPLDIRVGLIGSCTNSSYEDMGRSA-AVAKQALAHGLKC--KSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKN----------TIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTG-KDGKKF--KLEAPDADELPRAEF---D------------PGQDTYQHPPKDSSGQRVAVSPTSQRLQLLEPFDKW-----DGKDLEDLQILIKVKGKCTTDHISAAGP---------WLKF--------------RGHLDNISNNLLIGAINIENRKA-----NSVRNAVTQEFGPVPDTARYYKQHGIRWVVIGDENYGEGASREHSALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNK--IHPVDKLTIQGLKD-FAPGKPLKCIIKHPNGTQETILLNH-TFNETQIEWFRAGSALNRMKELQQ---

General information: TITO was launched using: RESULT: Template: 1C96.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4933 28301 5.74 38.82 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 5.74 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_1C96.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1C96-query.scw PDB file : Tito_Scwrl_1C96.pdb: