Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------MNTYVVDDSKYKCIYAGTEKAAAFNNEFENGTRVRVWFEGHHIKTFEKEQREVCGVGEWIVKYDAATELQKEVNRLEKTYFKKKELLDTIRQADGVGREINEKKKSILNYSGC------------------------------------------------ 1AER Chain:A ((1-211)) AFLGDGGDVSFSTRGTQNWTVERLLQAHRQLEERGYVFVGYHGTFLEAAQSIVFGGVRAAIWRGFYIAGDPALAYGYAQDQEPDA-RGRIRNGALLRVYVPRSSLPGFYRTSLTL-----------AAPEAAGEVERLIGHPLPLR--LDAITGPEEEGG----RLETILGWPLAERTVVIPSAIPTDPRNVGGDLDPSSIPDKEQAISALPDYASQPGKPPR

General information: TITO was launched using: RESULT: Template: 1AER.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 285 8271 29.02 87.06 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 29.02 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.061

(partial model without unconserved sides chains): PDB file : Tito_1AER.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AER-query.scw PDB file : Tito_Scwrl_1AER.pdb: