Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRYPNGKTFQPKHSVSSQNSQKRAPSYSNRGMTLEDDLNETNKYYLTNQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAYFKQSSTTDYNGIYKGRYIDFEAKETKNKTSFPLQNFHDHQIEHMKQVKAQDGICFVIISAFDQVYFLEADKLFYFWDR---KEKNGRKSIRKDELEETAYPISLGYAPRIDYISIIEQLYFSPSSGAKG 2OST Chain:A ((1-148)) -----MGST-KLKGDIAQQAAIMRALKM---GWGVLKPLGDRLSYDLVFDV---EGILLKVQVKSSWKSEKT----------------------GNYVVDNRRTRTNRRNIVRSPYRGNDFD----------FAVAYVEELELFYVFPVDVFISYGSEIHLVETDKRQRKPRSFGYREAW-----------------HLILQKGAAQKE

General information: TITO was launched using: RESULT: Template: 2OST.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 600 -35860 -59.77 -247.31 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -59.77 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.226

(partial model without unconserved sides chains): PDB file : Tito_2OST.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OST-query.scw PDB file : Tito_Scwrl_2OST.pdb: