Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEQPIGVIDSGVGGLTVAKEIMRQLPKENIIYVGDTKRCPYGPRPEEEVLQYTWELTNYLLENHHIKMLVIACNTATAIALDDIQRSVGIPVVGVIQPGARAAIKVTDNQHIGVIGTENTIKSNAYEEALLALNPDLKVENLACPLLVPFVESGKFLDKTADEIVKTSLYPLKDTSIDSLILGCTHYPILKEAIQRYMGEHVNIISSGDETAREVSTILSYKGLLNQSPIAPD--HQFLTTGARDQFAKIADDWFGHEVGHVECISLQEPIKR 2JFP Chain:B ((24-278)) --QEAIGLIDSGVGGLTVLKEALKQLPNERLIYLGDTARCPYGPRPAEQVVQFTWEMADFLLKKR-IKMLVIACNTATAVALEEIKAALPIPVVGVILPGARAAVKVTKNNKIGVIGTLGTIKSASYEIAIKSKAPAIEVTSLACPKFVPIVESNQYRSSVAKKIVAETLQALQLKGLDTLILGCTHYPLLRPVIQNVMGSHVTLIDSGAETVGEVSMLLDYFDIAH-TPEAPTQPHEFYTTGSAKMFEEIASSWLGIE---------------

General information: TITO was launched using: RESULT: Template: 2JFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1545 -204795 -132.55 -809.47 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -132.55 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_2JFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JFP-query.scw PDB file : Tito_Scwrl_2JFP.pdb: