Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMTSRKLSDILKQRLQHENRSFLFDREKDTLRVEDQTTKKGITLDLPPIIAKWELKKDEAIDEIVYYVSEAMTAMEGKAQEMTGKETRIYPVIRSTSFPDKSSEDIPLIYDDHTAETRIYYALDLGKTYRLIDQRMLEKENWTKERIR---ETAAFNLRSLPTVVKEDTVAGNYFYFFRANDGYDASRILNEAILNEYKQHAEGELAISVPHQDVLILADIRNESGYDILGQMSMSFFAGGTVPITALSFLYNEGKLEPVFILAKSRPKKD 1ZHH Chain:B ((15-235)) --RTKQQTSALIHNIFDSHFAAIQIHHDSNS-------------------------KSEVIRDFYTDRDTDVLNFFFLSIDQSDPSHT---PEFRFLTDHKG------IIWDDGNA---HFYGVN-DLILDSLANRVSFSNNWYYINVMTSIGSRHMLVRRVPILDPSTGEVLGFSFNAVVLDN-------NFALMEKLKSESNVD-NVVLVANSVPLANSLIGDEPYNVADV------------LVIETPIVVNAVTTELCLLTVQD----

General information: TITO was launched using: RESULT: Template: 1ZHH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 914 -10398 -11.38 -50.72 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -11.38 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.292

(partial model without unconserved sides chains): PDB file : Tito_1ZHH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZHH-query.scw PDB file : Tito_Scwrl_1ZHH.pdb: