Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIESTQELEKAVKDDWSVFM-FSADWCPDCRFVEPFLPELEANF-PEFTYYYVDRDKFIDTCAEWEIYGIPSFVVFNEGKEVNRFVSKDRK--TKEEIEQFLTDSLAKA 4BA7 Chain:B ((13-106)) ----------EEVLKSDKPVLVDFWAPWCGPCRMIAPIIEELAEEYEGKVKFAKVNVDENPETAAKYGIMSIPTLLLFKNGEVVDKLVGARPKEALKERIEKHL-------

General information: TITO was launched using: RESULT: Template: 4BA7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 363 -11699 -32.23 -129.98 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -32.23 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.629

(partial model without unconserved sides chains): PDB file : Tito_4BA7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BA7-query.scw PDB file : Tito_Scwrl_4BA7.pdb: