Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGSMDQSIAVKSPLTYAEALANTIMNTYTVEELPPANRWHYHQGVFLCGVLRLWEATGEKRYFEYAKAYADLLIDDNGNLLF--------------RRDELDAIQAGLILFPLYEQTKDERYVKAAKRLRSLYGTLNRTSEGGFWHKDGYPYQMWLDGLYMGGPFALKYANLKQETELFDQVVLQESLMRKHTKDAKTGLFYHAWDEAKKMPWANEETGCSPEFWARSIGWYVMSLADMIEELPKKHPNRHVWKNTLQDMIKSICRYQDKETGLWYQIVDKGDRSDNWLESSGSCLYMYAIAKGINKGYLDR-AYETTLLKAYQGLIQHKTETSEDGAFLVKDICVGTSAGFYDYYVSRERST--NDLHGAGAFILAMTELEPLFRSAGK 4Q88 Chain:A ((13-358)) ---------SEELLEIIHRVNQRYQSMHK-----PQVRSFWDNAVYHTGNMEVCALTGNKDYLQYSIDWADYNQWK-GATSNNKAEWRSTYGESPEYVLFGDWQVCFQTYADLYKLDPDTIKIARAREVMEYQMS---TSKLD--------YWHWVDALYMVMPVMVKMYDITKNPLYLEKLHQYLTVTDSIMYDREVGLYYRDAKYPY--PFH-KTINGKKDFWSRGDGWAFAAFAKVLKQLPAEDKYREEYITRFQTMAKSIAACQQP-EGYWTRSLIDPEQ-APGPETSGTALFTYGFLWGINNGLLNADIYSPIVEKAWNYLKTTALQ--ADGKIGYIQPV-GEKAI-P----GQVVDANSCFNFGIGAFLLAACERVRFL-----

General information: TITO was launched using: RESULT: Template: 4Q88.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1703 14923 8.76 45.36 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 8.76 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_4Q88.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q88-query.scw PDB file : Tito_Scwrl_4Q88.pdb: