Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMQEETFYSVRMRASMNGSHEDGGKHISGGERLIPFHEMKHTVNALLEKGLSHSRGKPDFMQIQFE---------EVHESIKTIQP--------LPVHTNEVSCPEEGQKLARLLLEKEGVSR------DV-----IEKAYEQIPEWSDVRGAVLFDIHTGKRMDQTKEKGVRVSRMDWPDANFEKWALHSHVPAHSRIKEALALASKVSRHPAVVAELCWSDDPDYITGYVAGKKMGYQRITAMKEYGTEEGCRVFFIDGSNDVNTYIHDLEKQPILIEWEEDHDS 1QFE Chain:A ((1-252)) -MKTVTV---------------KNLIIGEGMPKIIVSLMGRDINSVKAEALAYREATFDILEWRVDHFMDIASTQSVLTAARVIRDAMPDIPLLFTFRSAKEGGEQTITTQHYLTLNRAAIDSGLVDMIDLELFTGDADVKATVDYAHAHNVYVVMSNHDFHQTPSAEEMVSRLRKMQALGADIPKIAVMP--QSKHDVLTLLTATLEMQQHYA----------DRPVITMSMAKEGVISRLAGEVF-----GSAATFGAVKQASAPGQIAVNDLRSVLMILHNA--

General information: TITO was launched using: RESULT: Template: 1QFE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1211 17252 14.25 77.02 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 14.25 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_1QFE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QFE-query.scw PDB file : Tito_Scwrl_1QFE.pdb: