Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKICVVGAGYVGLTLSAALASIGHDMICTDKDVKKIGQLKKGVIPFYEPGLSDAILR---CGNLSFSSEVKSSMEECPVIFIAVGTPPRSDGSADTKALQSVIGDLSEAIRSYKTIITKSTVPPGTNENI----AKQLIASGVSKNLFNIVSNPEFLREGNALYDMLHPDKTVIGVQEEDHVSAAIVKSIYKHIDTPF-------IVTSLAGAELIKYANNFFLAAKISFINEMARICEAYQSDISDISRAIGLDPRIGKHFLQAGIGYGGSCFPKDLQALQFAAQEKNTETFLLRAVQHINDTQLGLYIKKIQSFF-ETLQGKKAAVLGISFKPNTDDIRNSQAVRLMERLAELGCDVHAYDPEA------VLPEHLRQH---------VTQHSQAFDAIEESDFLFLATEWSEFLAFDWKKAADIMKGRLVIDGRNVLKKELIEACGLICTGVGRP 2Y0C Chain:C ((9-460)) MNLTIIGSGSVGLVTGACLADIGHDVFCLDVDQAKIDILNNGGVPIHEPGLKEVIARNRSAGRLRFSTDIEAAVAHGDVQFIAVGTPPDEDGSADLQYVLAAARNIGRYMTGFKVIVDKSTVPVGTAERVRAAVAEELAKRG---QMFSVVSNPEFLKEGAAVDDFTRPDRIVIGC--DDDVPGERARELMKKLYAPFNRNHERTLYMDVRSAEFTKYAANAMLATRISFMNELANLADRFGADIEAVRRGIGSDPRIGYHFLYAGCGYGGSCFPKDVEALIRTADEHGQSLQILKAVSSVNATQKRVLADKIVARFGEDLTGRTFAIWGLAFKPNTDDMREAPSRELIAELLSRGARIAAYDPVAQEEARRVIALDLADHPSWLERLSFVDDEAQ---AARDADALVIVTEWKIFKSPDFVALGRLWKTPVIFDGRNLYEPETMSEQGIEYHPIGRP

General information: TITO was launched using: RESULT: Template: 2Y0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2215 -7325 -3.31 -17.44 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.83 3D Compatibility (PKB) : -3.31 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_2Y0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y0C-query.scw PDB file : Tito_Scwrl_2Y0C.pdb: