Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MNAVVIAVLLMLVLSLLRVNIVIALIIGALAGGLTGGLGLGETVKAFTDGLGGNATVAVSYAMLGAFAAALTKTGLPDAMVEASVKLIGNKEDSRKKALSKVLIVLIILIVSCFSQNVVPVHIA--FIPVLIPP--LLKIFNELEMDRRLIACVITFGLTAPYILLPVGFGQIFQGMLKDNMADAGLNVPLADIPYALIIPVAGMVVGLILSVIVYRKPKQYETKDISGAEASPYTRKSIGIAV-LAIVVSLGVQLYLSQTLGVEGMIMGALA-----GLIVLFVSGVM-KRDEADSLITDGMVLMAFIGFVML-V--AAGFSNVLTKTGDVESLVKT-SAGFIGHSQSLGALLMLIVGLLITMGIGSSFATIPVITTIFVPLCMQLGFSPMATIAIIGAAAALGDAGSPASDSTLGPTSGLSADGQHHHIWDTCVPTFIFYNIPLVIFGWIAALVL 4KK0 Chain:A ((2-431)) PLQSLVKALWNVLHEPDL--------------------TELIAEVESYQQRYP-KQNPTNSQKIRHILDEIYEKTPFNN--------------TRRRILWLAVLKTVIPLLI--LDRQAVGEWWDQIFFPFLNSPTQLKPVFSDL---KSILFYILIFHDEDEW---GGDLRRECAEETITRLVDLYVSKAIENLESQEQRNQTIECLVNVLVHYGIQRPKEL-SSCFCHHFLNPPTRIPI-LSVMVEVIRRQGPRLYEIPQTGFYDLVLKCAEFDTSPILLSYALSFILMILSHICNSLDDSLYRLFCIYLRFSMIDPTSGFPSS-TASGNWEVFHDFMSTGSSQPDYLESLDYSQLFSILYALYPINFLEFLRDPKLYASKHNFQIRYSFNQELLSTKSDGLLGR--HLAHSNFLKYTAETELTDKSRWTRLDSIAVVALCNSLNAV---------

General information: TITO was launched using: RESULT: Template: 4KK0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1652 -114663 -69.41 -297.83 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -69.41 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_4KK0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KK0-query.scw PDB file : Tito_Scwrl_4KK0.pdb: