Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------MKFVKAIWPFVAVAIVFMFMSAFKFNDQLTDQEKQKIDMEMNKIQQQEEPVNANK---------------------- 2IVY Chain:A ((2-89)) AMLYLIFYDITDDNLRNRVAEFLKKKGLDRIQYS-----VFMGDLNSSRLKDVEAGLKIIGNRKKLQEDERFFILIVPITENQFRERIVIGYS

General information: TITO was launched using: RESULT: Template: 2IVY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 131 9059 69.15 181.18 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : 69.15 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.664

(partial model without unconserved sides chains): PDB file : Tito_2IVY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IVY-query.scw PDB file : Tito_Scwrl_2IVY.pdb: