Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREELKGIFASVFGEKEGLRFFFAPGRVNLIGEHTDYNGGHVFPCALTMGTYAAVAERNDGLVRMYSDNFRNAGIKECSLDDIRYQKEDDWANYPKGVIYEFQQRGYAVPHGFDIVFSGNIPNGAGLSSSASIELLMGVVLQSYFHPEVDALELVKMAQHAENHFIGVNCGIMDQFAIGMGKKHHAMLLNCDTLDYEYSKLNVSGLALVIANTNKKRTLADSSYNTRRQECNDALLDLKKGLDIAALGDIKPSDFDAHSSLIQNETNRRRAKHAVYENHRAIKTAHMFKENNIDEIGQLMKESHLSLKDDYEVTCPELDELVFAAWDHEGVIGSRMTGAGFGGCTISIVKDEFVDDFIQKVGDRYQEKTGLRADFYVADIGEGARELKGE 1H74 Chain:A ((1-296)) MKVRVKAPCTSA-------------------------------NLGVGFDVFGLCLKEPYDVIEVEAIDDKEIIIEV----DDKNIPTDPDKNVAGIVAKKMIDD-FNIGKGVKITIKKGVKAGSGLGSSAASSAGTAYAINELFKLNLDKLKLVDYASYGELASSGA--KHADNVAPAIF----------------------GGFTMVTNYEPLEVLHIP----------------IDFKLDILIA---IPNISINTKEAREILPKAVGLKDLVNNVGKACGMVYALYNKDKSLFGRYMMSDKVI-EPVRGKLIPNYFKIKEEVKDK--VYGITISGSGPS--IIAFPKEEFIDEVENILRDYYEN-------TIRTEVGKGVEVV---

General information: TITO was launched using: RESULT: Template: 1H74.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1834 -148517 -80.98 -501.74 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -80.98 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_1H74.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H74-query.scw PDB file : Tito_Scwrl_1H74.pdb: