Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MLKKIALTLCPAIVGSLLFFTAP-A---------SAHVSVKPA-------------ESAAGSWETYTMKVPSEKNLPTTKVVLKMPK-DVEFQQYEPIPGWKVSTQKHDDKSVSVTWEATDGGIQEGQFQQFT-----------------FVAKNPDKAEEAAWDAYQYYKDGSIVE-----WTGDEDADTPHSITNITSAKQV-TDEHGATKTEDDSE-NSGSSALDITAMVLSAAAIILSVAALVKK-KRA-------------------------------------------- 4EI0 Chain:A ((12-311)) PVSALSNDCIKRSLPVAPNIVGNEIEFAYAMAIPNELGKLSSAQVVSSIAGATGTYFDPNSYYTNSSGQDIPVKVCSDSQTNGTTTVIDFTVDTCAATLRYYYIIP----EEARGKDVQFSFSVKASNGQVAEYKLGPYKISKMDMAKNLSVTNDKCYLSFLNEGEAVHIYSKADLQANPSLAAKIDIMYAYSEKSDLSHAFYTSSSPKEYMGGTELPSGFVNNTKMIKVYGLQDRQLSDLQYSKFIDDLDFETIDMSKCTNYILGLKEEAGAWVETADGKYRAYVYINKASASEVTVSVKRYK

General information: TITO was launched using: RESULT: Template: 4EI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 660 -35806 -54.25 -179.03 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -54.25 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_4EI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EI0-query.scw PDB file : Tito_Scwrl_4EI0.pdb: