Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLDEFTIGQVFKT-KSLKVSKDDIMRFAGE-FDPQYMHVDEEKAS-KGRFNGIIASGIQTLAISFKLWIEEGFYGDDIIAGTEMNHMTFIKPVYPDDELFTIVEVLDKQPKRN-----ELGILTVLLSTYNQKEVKVFEGELSVLIKR 2BI0 Chain:A ((13-157)) PYFDDLSKGQVFDWAPGVTLSLGLAAAHQSIVGNRLRLALDSDLCAAVTGMPGPLAHPGLVCDVAIGQSTL--AT-QRVKANLFYRGLRFHRFPAVGDTLYTRTEVVGLRANSPKPGRAPTGLAGLRMTTIDRTDRLVLDFYRCAMLP-

General information: TITO was launched using: RESULT: Template: 2BI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 634 -76659 -120.91 -559.55 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -120.91 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_2BI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BI0-query.scw PDB file : Tito_Scwrl_2BI0.pdb: