Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGTEKVTALKAKHQSKSSTGLSWFERIGYGFGDMSYNIIFQFVNAYLLFYYTDVGGIQPAVIATLFLVVRVLDAIFDPIMGLILDKTNTRWGKARPYLLWVAFPFALFTFLCFTTPHFG-ETGNMVYAYVTYILLGMSFSMQTIPVNSLTGRMTNSVEERTVLTTTRMILVYIGILLSISCATPLATAIGG-------EDQAFGFQMTALIYAAVSIVLNLFSFFTVRERIQPKKRKKQGIKKTLSV-LFKN----KPLLMLISSFLAFAIGFNIKLSTMVYYFTYNVNHKE-F---------VF-MGTVLFFGAALISNLFIPFFSEKWGRKQVMIITAALSLIS-YAGLHFTPY-SSIPLIFIWLF-ASGFFTTPLNTLAWGMVADCVDYAEWKTGIRADGVVISSMSFINKLGVALAGSFSAIYLGIAGYVANTDQTVASLNAIKNMNALIPGFFILLSIILIAFYPLTEKRYKHIISELEQRSAR 4J05 Chain:A ((68-479)) ------------------------------------------------------KTGLPAQRQDLMKAAANIGCVVGQVMFGVLGDS----FGRKFVYG-KELILIIVATIFQMSAPSHWDGNRVLTWITICRVFLGIGIGGDYPMSATVVSDRANI-HRRGTLLCFIFANQGWGSFVGSLVTIVTISGFKHRLKSGHTHDVDKAWRILIGLSLIPAFGTL-YQR-----------------WQE-FVAYFSTWNHFRNLLGSMLGWFLVDIAFYGINLNQSVVLAQI-GFAGKTGDVYDKLFQLATGNIIVTALGFLPGYYFTLFLIDIVGRKKLQFMGFIMSGLFLAILAGEIDHIGKGP-LLACFTFMQFFFNFGANTTTFIVAAELFPT-------RIRASAHGISAAAGKCGAILSSLVFNQLKAKIG---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4J05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1362 -195315 -143.40 -614.20 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -143.40 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.296

(partial model without unconserved sides chains): PDB file : Tito_4J05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J05-query.scw PDB file : Tito_Scwrl_4J05.pdb: