Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLPELQRSITWIQGTALTIGAVLGC----GILILPSVTADT----AGPASLFVWV-------FMSF-------------LSFFLVGT----LARLVKIAPSAGGITAYVQLAFQKKAGAIL--GWIMLGSVPIGVPIIALTGAHYVSYITEAADWQITLIA-----GCMLAISILLHMRGIQLSANISTLVICVIVFLLVTSIAVSLPHVTIAEFKPFLPHGWSAAGSVSVMIFFSFVGWEMI-------------------------TPLAEE---FHRPEKDVPLSLFLAASCVAG---LYIMLSF----------VTVGTHS----------YGENGEIASLAMLISKGAGESGVYVTVC-LALFITFATIHANIAG-FSRMVYALAREGHIPVFFGKLSATK--RTPIRVLTAMAAVFG------LVLAAHGLFQIDLTTLLKGPSAAFIASYICTMAAA-----LKLLGRRDIGWWMAL---GAFVACAVIYSFSGWALLYPAVLAAAGYFYMKTKGGHKKKLDHVL------ 1N7V Chain:A ((1-555)) ANFNVPKLGVFPVAAVFDIDNVPEDSSATGSRWLPSIYQGGNYWGGGPQALHAQVSNFDSSNRLPYNPRTENNPAGNCAFAFNPFGQYISNISSAQSVHRRIYGIDLNDEPLFSPNAASITNGGNPTMSQDTGYHNIGPINTAYKAEIFRPVNPLPMSDTAPDPETLEPGQTEPLIKSDGVYSNSGIASFIFDRPVTEPNPNWP-PLPPPVIPIIYPTPALGIGAAAAYGFGYQVTVYRWEEIPVEFLNPEGSPCAYEAGIILVRQTSNPMNAVAGRLVPYVEDIAVDIFLTGKFFTLNPPLRITNNYFADDEVKENTVTIGNYTTTLSSAYYAVYKTDGYGGATCFIASGGAGISALVQLQDNSVLDVLYYSLPLSLGGSKAAIDEWVANNCGLFPMSGGLDKTTLLEIPRRQLEAINPQDGPGQYDLFILDDSGAYA-SFSSFIGYPEAAYYVAGAATFMDVENPDEIIFILRNGAGWYACEIGDALKIADDEFDSVDYFAYRGGVMFIGSARY---TEGGDPLPIKYRAIIPGLP

General information: TITO was launched using: RESULT: Template: 1N7V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2079 -287364 -138.22 -685.83 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -138.22 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.054

(partial model without unconserved sides chains): PDB file : Tito_1N7V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N7V-query.scw PDB file : Tito_Scwrl_1N7V.pdb: