Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTLTVKDIKDRLQEVKDAQDPFIAQCENDPRKSVQTLVEQWLKKQAKEKALKEQWVNMTSYERLARNKGFRLIAGVDEVGRGPLAGPVVASAVILPEE----CEILGLTDSKKLSEKKREEYYELIMKEALAVGIGIVEATVIDE----INIYEASKMAMVKAIQDLSD------TPDYLLVDAMTLP--------------------LDTAQASIIKGDAKSVSIAAGACIAKVTRDRMMSAYAETYPMYGFEKNKGYGTKE-HLEAL----AAYG-PTELHRKTFAPVQSFR 1EKE Chain:A ((2-218)) ------------------------------------------------------------------------IIIGIDEAGRGPVLGPMVVCAFAIEKEREEELKKLGVK---ELTKNKRAYLKKLLENL-GYVEKRILEAEEINQLMNSINLNDIEINAFSKVAKNLIEKLNIRDDEIEIYIDACSTNTKKFEDSFKDKIEDIIKERNLNIKIIAEHKADAKYPVVSAASIIAKAERDEIIDYYKKIYGDI----GSGYPSDPKTIKFLEDYFKKHKKLPDIARTHWKTCKRI-

General information: TITO was launched using: RESULT: Template: 1EKE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 874 -107729 -123.26 -619.13 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -123.26 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_1EKE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EKE-query.scw PDB file : Tito_Scwrl_1EKE.pdb: