Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MKIDYVSDLHINHWIPWNVNQIKWEKRTREIVNRLISNGNG-EVLVIAGDFT-----EWNQQTLWVLDEAAKQYE-KVYFTYGNHDLYLLSKSQKRKYSDSLGRLNDLIQKAADMKNVTPLIKTTETYKGKVFAGDVMWYLPKGIEGWDFFKGVSNDSNYIWLNGYNKVDGVRAMWKESMDWYETLENTQVDVFVSHVPPVHNPYSPFEPNTC-----YMVDVPFINAKHWVCGHDHLQAEFDKDGTSFHMNCIGYPYDYDNYPSVNVIPGEEVDSYKTFELKTFEI 2HY1 Chain:A ((12-267)) PRPDYVLLHISDTHLI----------DADDRLGELLEQLNQSGLRPDAIVFTGDLADKGEPAAYRKLRGLVEPFAAQLGAELVWVMGNHD-----------------DRAELRKFLLDEAPSMAPLDRVCMIDG--------------------LRIIVLDTSVPGHHHGEIRASQLGWLAEELA----TPAPDGTILALHHPPIPSVLDMAVTVELRDQAALGRVLRGTDVRAILAGHLHYSTNATFVGIPVSV-----ASATCGCNLVHVYPDTVVHSVIP--------

General information: TITO was launched using: RESULT: Template: 2HY1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1161 20714 17.84 98.17 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 17.84 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_2HY1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HY1-query.scw PDB file : Tito_Scwrl_2HY1.pdb: