Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVTYWTGENLTLRAIQPEDIVIFDSLDDEI-LRNMDSLHFPRSANNMREWVEEQL-EKDEFRFIAVESDNNIVGMIETFDCDRKNGTFGYYLAVFEPYRGKGFAKEMILMVLRFFFLELAYQKVNTTVYSFNNPSIRLHEKLGFMKEGQLRKIIFT-KGAYYDGICFGMTREEFELNHG 2Z10 Chain:A ((4-180)) -FPERFEGRHVRLEPLALAHLPAFLRHYDPEVYRFLSRAPVAPTEEALRAHLEGLLGEPGRVNWAILF-GKEVAGRISVIAPEPEHAKLELGTMLFKPFWGSPANKEAK-LLLRHAFEVLRAERVQFKVDLRNERSQRALEALGAVREGVLRKNRRLPDGAFRDDVVYSVLKEEWPGVK-

General information: TITO was launched using: RESULT: Template: 2Z10.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 797 -18833 -23.63 -108.86 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -23.63 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_2Z10.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z10-query.scw PDB file : Tito_Scwrl_2Z10.pdb: