Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGYNIPRGTQDILPGESDRWQFVEQIMRDTCRTYQYKEIRTPIFEHTELFARGVGESTDIVQKEMYTFEDRKGRSLTLRPEGTAAAVRAFNENKLFANPVQPTKLYYVGPMFRYERPQTGRYRQFYQFGIEAIGSKDPAIDAEVMALAMSIYEKAGL-ENVKLVINSLGDQDSRKSYREALVKHFEPRIEEFCSDCQSRLHTNPLRILDCKKDRDHELMKSAPSILTYLNEESAAYFEKVKQYLNDLGISYEIDPNLVRGLDYYNHTAFEIMSNAEGFGAITTLAGGGRYDGLVEQIGGPEAPGIGFAMSIERLLAAIDAEKRELPVDKGIDCYIVTLGEKAKDYSVSLVYKLRE--AGISSEIDYENKKMKGQFKTADRLKARFIAILGEDELAQNKINVKDAQTGEQIEVALDEFIHVMKANQKG 1HTT Chain:C ((8-412)) ------RGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLPIVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYN--QEQRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEHVTLELNSIGSLEARAN---------------------------------------------------YLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTN------QGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLVASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEVANGTAVVKDLRSGEQTAVAQD------------

General information: TITO was launched using: RESULT: Template: 1HTT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1659 -30946 -18.65 -89.18 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -18.65 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_1HTT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HTT-query.scw PDB file : Tito_Scwrl_1HTT.pdb: