Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKAISIFPKEFGFFPYIFLVYTIMPFLSLLKESGVKQGIGYGMLLLFVAAYRQLFCSVGKASFTYWLIVQMAVILMYSVFYNITYIYLGFFPANFVGYYKEKTNFNRAFCALIFILLFPCLYQFIANSVSLRELFSVLPFLVIMLISPFGIRSMFRRIELEAKLAQANEQIKELSKREERVRIARDLHDTLGHTLSLLTLKSQLIQRLAASDPERTKLEAKEMETSSRSALKQVRELVSD-----------MRTVTITEELVNIQHIL----RAG-NITFQYEGADDFSVISPVTQNIISMCMREAVTNIIKHSKA---------THCAITISQFADKMRIVIRDDGKGAPKE-------------KMFGNGLWGMEERLMLIEGGLTVSD--HNGTVVALTIPLIKKAE 4E01 Chain:A ((146-405)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIE-DEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTA-GFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDG--

General information: TITO was launched using: RESULT: Template: 4E01.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 603 -28471 -47.21 -148.28 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : -47.21 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_4E01.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E01-query.scw PDB file : Tito_Scwrl_4E01.pdb: