Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRNRLKKNEDFQKVFKHGTSVANRQFVLYTL-DQPENDELRVGLSVSKKIGNAVMR--NRIKRLI------RQAFLEEKERLKEKDYIIIARKPASQL--TYEETKKSLQHLFRKSSLYKKSSSK 4WBE Chain:A ((132-251)) --RREQLMREEAEQKRLK---TVLELQYVLDKLGDDEVRTDLKQGLNGVPILSEEELSLLDEFYKLVDPERDMSLRLNEQYEHASIHLWDLLEGKEKPVCGTTYKVLKEIVERVFQ--SNYFDSTHN

General information: TITO was launched using: RESULT: Template: 4WBE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 278 -1512 -5.44 -13.87 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -5.44 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_4WBE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WBE-query.scw PDB file : Tito_Scwrl_4WBE.pdb: