Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDS-----IPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM 4FKV Chain:A ((4-297)) MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRL-----GVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIP------LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLAR-----------EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHL

General information: TITO was launched using: RESULT: Template: 4FKV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) 32104 for 2151 contacts (14.9/contact) + 2D Compatibility (PS) -27291 + (NN) 7288 + (LL) 1272 1D Compatibility (HY) 17600 + (ID) 450 Total energy: 30523.0 ( 14.19 by residue) QMean score : 0.091

(partial model without unconserved sides chains): PDB file : Tito_4FKV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FKV-query.scw PDB file : Tito_Scwrl_4FKV.pdb: