Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKLNEALKKAERIVFLTGAGVSVPSGIPDYRSKNGLYAGMSSPEYMLSHTCLVREPEKFYQFVTENMYYPNAVPNVIHKKMAEIETEKDV-TIITQNIDGLHEKAGSKKVVNFHGSLYHCYCQNCGMTVT----AKDYLKSDIH--TDCGG-VIRPDVVLYEEAISESAIDQSLTAIRKADLIVIVGTSFRVSPFCNLTDYRNKK--ARIFAVNKERISLPYPFEMMESDAVKVFEEI 4TWJ Chain:A ((5-219)) IRKAAEILAKSKHAVVFTGAGISAE------------------PEEVASISGFKRNPRAFWEFSMEMKDKLFAEPNPAHYAIAELERMGIVKAVITQNIDMLHQRAGSRRVLELHGSMDKLDCLDCHETYDWSEFVEDFNKGEIPRCRKCGSYYVKPRVVLFGEPLPQRTLFEAIEEAKHCDAFMVVGSSLVVYPAAELP-YIAKKAGAKMIIVNAE----------------------

General information: TITO was launched using: RESULT: Template: 4TWJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -108610 for 1427 contacts (-76.1/contact) + 2D Compatibility (PS) -20573 + (NN) -10223 + (LL) 2132 1D Compatibility (HY) -10800 + (ID) 3400 Total energy: -151474.0 ( -106.15 by residue) QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_4TWJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TWJ-query.scw PDB file : Tito_Scwrl_4TWJ.pdb: