Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQLHRRMGTFSLMMVGLGSMIGSG----WLFGAWRAAQIAGPAAIISWVIGMVVILFIALSYSELGSMFPEAGGMVKYTQYSHGSFIGFIAGWANWIAIVSVIPVEAVASVQYMSSWPWEWAKWTSGLVKNGTLTGEGLAFASVLLLIYFLLNYWTVNLFSKANSLITIFKIIIPGLTIGALLFVGFHGENFTGGQSIAPNGWASVLTAVATSGIVFAFNGFQSPINMAGEAKNPGKSIPIAVVGSLFVATVIYVLLQIAFIGAVNPSDIAHGWSHLNFNSPFADLAIALNINWLVIVLYADAFVSPSG-TGITYTATTSRMIYGMEKNKYMPSIFGKLHPIYGVPRQAMFFNLIVSFIFLFLFRGWGVLAEIISVATLISYITGPITVMTLRRTGKDLYRPLRLKGLNVIAPLGFIFASLVL----YWARWPLTGQVLFIILIGLPIYFYYQAKAKWKGFGRNFKAGVWMVFYLLAMMVISYLGSDKFGGLNVIHYGWDMVLIAMVSLVFYVWALKSGYQTEYLKDAKEINSQLLNGQSEAAAGKE 4BWZ Chain:A ((3-386)) -GAEHLLEIFYLLLAAQVCAFIFKRLNQPVVIGEVLAGVLVGPALL---------------------------------------------------------------------------------GLVHEGEILEFLAELGAVFLLFMVGLETRLKDILAVGKEAFLVAVLGVALPFLGGYLYGLEIGF----------------------------------------------ETLPALFLGTALVATSVGITARVLQELGVLSR-------------PYSRIILGAAVIDDVLGLIVLACVNGVAETGQVEVGAITRLIVLSVVFVGLAVFLSTL--IARLPLERLPVGSPLGFA-LALGVGMAALAASIGLAPIVGAFLGGMLLSEVREKYR-LEEPI-FAIESFLAPIFFAMVGVRLELSALASPVVLVAGTVVTVIAILGKVLGGFLGALTQGVRSALTVGCGMAPRGEVGLIVAALGLKA-GAVNEEEYAIVLFMVVFTTLFAPFALKPLIAWTERERAAKE-----------------

General information: TITO was launched using: RESULT: Template: 4BWZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2085 -402504 -193.05 -1073.34 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -193.05 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_4BWZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BWZ-query.scw PDB file : Tito_Scwrl_4BWZ.pdb: