Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MRFFIADDDRAVRSILRQIIEDEDLGEAAGEADDGSQVEGHMLQFKQIDILLIDLLMPGRDGIETIRQIQNTYSGK---IVMISQVEAKEMVGEAYSLGIEYFIHKPINRIEIVTVLQKVKERIELEHSIGAIQHSL--------------------------SRLVNRTERKARPQQKSDSGLKEAGTFLLSE--------------------LGMMGEGGAHDLMAVLQYLAEHEQSEPHEKQSPSLKQIFT-----QVAVRKLGTGASQTEVNREMKAS----------------------EQRIRRAIIHSLHHFASLGTTDFSNPKFETYASKFFDFPVVSQKMKELQSKDAKPLAP----ARINMKKFIHVF--------FLEAKLLHETMKQRRI------------------------------------------------------- 1W25 Chain:A ((1-454)) SARILVVDDIEANVRLLEAKLTAEYY--EVSTAMDGPTALAMAARDL-PDIILLDVMMPGMDGFTVCRKLKDDPTTRHIPVVLITALDGRGDRIQGLESGASDFLTKPIDDVMLFARVRSLTRFKLVIDELRQREASGRRMGVIAGAAARLDGLGGRVLIVDDNERQAQRVAAELGVEHRPVIESDPEKAKISAGGPVDLVIVNAAAKNFDGLRFTAALRSEERTRQLPVLAMVDPDDRGRMVKALEIGVNDILSRPIDPQELSARVKTQIQRKRYTDYLRNNLDHSLELAVTDQLTGLHNRRYMTGQLDSLVKRATLGGDPVSALLIDIDFFKKINDTF-GHDIGDEVLREFALRLASNVRAIDLPCRYGGEEFVVIMPDTALADALRIAERIRMHVSGSPFTVAHGREMLNVTISIGVSATAGEGDTPEALLKRADEGVYQAKASGRNAVVGKAAH

General information: TITO was launched using: RESULT: Template: 1W25.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1198 -117670 -98.22 -379.58 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -98.22 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.380

(partial model without unconserved sides chains): PDB file : Tito_1W25.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W25-query.scw PDB file : Tito_Scwrl_1W25.pdb: