Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQEELTIHKALPADGDWKSLLFQPLPERDHYETAQGLSFSRLAGQILGTPIDETDYYNELYELSVN-------------DRITI---LSETLDKTIEPET-FQKLQHIHSINQKE---KGLSVSRFVAFLDGEKLIAKHSNPLMHRHLRKALMTLLHTFA--DSHEKGLNHPDFRRVLLD-VSKFSLNHLNPWLEKTDIEREMPKVVWY-----GDATKSQLYFLYYLMLVGCDVLLFHPAGTDQLALVDPKQELGFTEKLPDVSELQP-----FPKEKPDRKSTVAYRSTKEIEHVLNH------------------------EESMLYKPWQFRDHTPVSVTLKTTYDELFLITKERAFIRPNFKADKHSIEIPNVFAKIMGVSKDNKEYWNRLHTLAD-YQETEMVRSFPFTEEIKSNYQFHYSHALDQEGNIDPDKLMASNVWQYKQLPAGVQ-----TAIAKTISRMCRYPRLKALHQEQVKD----------VQIYLFKQTTNLPANLLK--LIQMFDYAQTV--PKLVLYHTEMSGGLTRSDA-AALLFLNEIGIDIIIYNPPGHQDIEQFIEEDQYDIHWLDDMVFQQD-YKEPSLVKRLFRTITQK--------------- 4NFU Chain:A ((15-633)) AFEALTGINGDLITRS-WSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSSFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAVIDPRTSFQASVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFVNFVTRFDIVPRITLARKASVEETLPHVLAQLDPRNSSVQESEQRITEFYTSVMRDTSTVANQAVCELTG-SAEAILETLSSFLELSPYRPAGTFVFSTEK----RLVAVNNSDAILQMLFYTCQASDEQEWSLIPFRSIRDHHSYEELVQSMGMKLFNHLDGENSIESSLNDLGVSTRGRQYVQAALEEEKKRVENQKKIIQVI----QQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLLKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHHILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNSGSECGSCFWAEV--EELKGKPYEEVEVRVKTLEGMLREWITAGEVDEKEIFLEGSTFRKWWITLPKNHKSHSPLRDYMMDEI

General information: TITO was launched using: RESULT: Template: 4NFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2353 -118534 -50.38 -225.78 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -50.38 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.108

(partial model without unconserved sides chains): PDB file : Tito_4NFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NFU-query.scw PDB file : Tito_Scwrl_4NFU.pdb: