Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDRLPRIPLADIIDRF-VDWITMTFGGFFDGIANGLAAFV-NGIVTGLGFIPSILLTIIFAALAWWISTRGIALFTLIGFLLIDYLGYWDPMLQTLALVLTSVIISIVVGVPIGIWASQKETVRRIVTPILDLMQTMPAFVYLLPAIFFFNIGVVPGVVASVIFAMPPTIRMTVLGIKQVPADLIEATEAFGSTTAQRLFKVQLPLATKTILAGINQSIMLALSMVVIAAMVGAPGLGSEVYSAVTQL-KTGVGVEAGIAIVIVAITLDRITQNIKVKKKSRGNA 2G7L Chain:A ((16-230)) KPALSRRWIVDTAVALMRA--------------EGLEKVTMRRLAQELDTGPASLY----------------------------------VYVANTAELHAAVLDALLGEVDLTGAEDWREQLRAVLTSYTLVLFAHPQLARSALV---------ARPSGENYLRLVERVLELLARSGAPGAQVAWGVDKLLQDATATAAEQATS-----------------------ATVRALRDADEATHPAIASHMPLLV---AGSAHDRLRWSFDVLVNGITRTPVPGPA-

General information: TITO was launched using: RESULT: Template: 2G7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 711 -136616 -192.15 -689.98 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -192.15 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_2G7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2G7L-query.scw PDB file : Tito_Scwrl_2G7L.pdb: