Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAENQEVYDVTIIGGGPIGLFTAFYCGMRELKTKVIEFLPKLGGKVSLFFPEKIIRDIGGIPGIAGKQLIEQLKEQAATFDPDIVLNQRVTGFERLDDGTIVLTGSEGKKHYTRTVILACGMGTLEVNEFDSEDAARYAGKNLH-YGVEKLDAFKGKRVVISGGGDTAVDWANELEPIAASVTVVHRREEFGGMESS-VTKMKQSSVRVLTPYRLEQLNGDEEGIKSVTVCHTESGQRKDIEIDELIINHGFKIDLGPMMEWGLEIEEGRVKADRHMRTNLPGVFVAGDAAFYESKLRLIAGGFTEGPTAVNSAKAYLDPKAENMAMYSTHHKKLVHK 4GCM Chain:A ((4-309)) ----EIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGI-PGGQMANT-EE--VENFPGFEMITGPDLSTKMFEHAKKFGAVYQYG-DIKSVEDK--GEYKVINFGNKELTAKAVIIATGA-E--YKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEGTFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTLTSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAGDVRDK-G-LRQIVTATGDGSIAAQSAAEYIEHLN----------------

General information: TITO was launched using: RESULT: Template: 4GCM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1804 -189242 -104.90 -622.50 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -104.90 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_4GCM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GCM-query.scw PDB file : Tito_Scwrl_4GCM.pdb: