Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIKRFKMKLGTKILCLVFVVILLFSASVGTVMLKEITESMKQMATEKAKGDLALSSTYIDDVMSGDWQVKNNKLYKGQTQINGNEDIVDLLGEKTGDTITIFQGDTRVATNVMKNGERAVGTQASSEVIAAVLKKGKRFYGQADVAGSSYQTAYMPLKDQNGNIIGMLYTGANQSILASLTQSLFTQFAIVLVIVIMVSVILVLVFTRKINKRLNAL-KSAFESAGNGDMTIEVSDKTGDELSELSVYYNKMRMKLNDTI---QTVQQSALQLASASQQLSAGAEETNQASEKITEAVQQIANGAQDQITRIENSESSLKQASADIRDISANTAAIADKGQLAQSKADIGQKEIANVQAQMDAIHQSIQKSGEIIHQLDGRSKQIEQILSVITQIADQTNLLALNAAIEAARAGEQGKGFAVVADEVRKLAEESQQSAGQISKLIIEIQKDMNRSARSVEHVKTEAAEGVTMIQRTRDAFKEIAAATGEISAEISDLSASVTNISASAHQINDSFAANTADIKESTKNTRQAAALTEEQFAAMEEITAASETLSQLAEELTGIISQFKMINQAENG 4I3M Chain:A ((10-85)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HAVAQQRADRIATLLQSFADGQLDTAVGEAPAPGYERHYDSLRALQRQLREQRAELQQVESLEAGLAEMSRQHEAG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4I3M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 96 -6649 -69.26 -92.34 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -69.26 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_4I3M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4I3M-query.scw PDB file : Tito_Scwrl_4I3M.pdb: