Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQYASESINLPGEITFKDVREIFFYQIAKISCFYFLLFCAIFAAVNFINGWPRIVYGSDALNLFMNSMLIIVMSVLFTLLLLLLLYVKFS------RAYKKNERMKSKRTYTLNQEGIRICSKKYDLIFNWDEITAVFEYKN---IFRVNTSSGQYIAIPKHFFHSEEEMNRFKEIILKNTETKKLKFKKDQH 2LU7 Chain:A ((1-82)) ------------------------------------------------------------------------MTRVRSTPGGDLELVVHLSGPGGPVRWYKDGERLASQGRVQLEQAGAR-QVLRVQGARSGDAGEYLCDAPQDSRIFLVSVEEP---------------------------------------

General information: TITO was launched using: RESULT: Template: 2LU7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 286 -35413 -123.82 -485.11 target 2D structure prediction score : 0.18 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -123.82 2D Compatibility (Sec. Struct. Predict.) : 0.18 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.330

(partial model without unconserved sides chains): PDB file : Tito_2LU7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LU7-query.scw PDB file : Tito_Scwrl_2LU7.pdb: