Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MGITKEEVNSYYQKAGIVLT-------DEEV----------DQIQLMDYGL----GKERKVGLQLFV-------------YVNTDRYCSKELVLFPGQTCPEHRHPPVDGQEGKQETFRCRYGKVYLYVEGEKTPLPK------------------VLPPQEDREHYTVWHEIELEPGG--QYTIPPNTKHWFQA---GEEGAVVTEMSSTSTDKHDIFTDPRI---------------------------- 4Q0S Chain:A ((12-260)) SARTSITRREYDEWVREAAALGKALRYPITEKMVNDSAGIVFGADQYDAFKNGMWSGEPYEAMIIFESLNEPAVDGLPTGAAPYAEYSGLCDKLMIVHPGKFCPPHHHGR------KTESYEVVLGEMEVFYSPTPSAESGVELLNFSGMPVGSPWPEGVALPKGRESSYEKLTSYVRLRAGDPKFVMHRKHLHAFRCPPDSDVPLVVREVSTYSHEPTEAAAGNHAPIPSWLGMHDNDFVSDAANTGRLQTAIS

General information: TITO was launched using: RESULT: Template: 4Q0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 730 -38758 -53.09 -240.73 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -53.09 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.346

(partial model without unconserved sides chains): PDB file : Tito_4Q0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q0S-query.scw PDB file : Tito_Scwrl_4Q0S.pdb: