Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTFFQFDELGTSYRNEIIGGLTTFLSMAYILFVNPITLALESVKDFPEALRIDQGAVFTATALASAAGCILMGLIARYPIAIAPGMGLNAFFAFSVVLGMGISWQAALSGVFISGLIFVALSLTGFREKIINAIPPELKLAVGAGIGLFITFVGLQGSGIITANPSTLVTIGNIHSGPVLLTI-FGVIVTVILMVLRVNAG---VFIGMLL-TAVAGMIFGLVPVPTQIIGSVPSLAPTFGQAWIHLPDIFSVQMLIVILTFLFVGFFDTAGTLVAVATQAGLMKENKLPRAGRALLADSSSIVIGAVLGTSTTTSYVESSSGVAAGARSGFAAIVTGILFLL----ATFFSPLLSVVTSNVTAPALIIVGALMVAPLG----KIAWDKFEV----AVPAFLTMIMMPLTYSIATGIAI--GFIFYPITMVCKGKAK-EVHPIMYGLFVVFI-----LYFIFLK 5DBJ Chain:D ((5-441)) ---------SDHDYDVVIIGGGPAGSTMASYLAKAGVKCAVFE-KELFEREHVGESLVPATTPVLLEIG--VMEKIEKANFPKKFGAAWTSADS-GPEDKMGFQ---GLDHDFRSAEIL-------FNERKQEGVDRDFTFHVDRGKFDRILLEHAGSLGAKVFQGVEIADVEFLSPGNVIVNAKLGKRSVEIKAKMVVDASGRNVLLGRRLGLREKDPVFNQFAIHSWFDNFDRKSATQSPDK----VDYIFIHFLPMTNTWVWQIPITETITSVGVVTQKQNYTNSDLTYEEFFWEAVKTRENLHDALKASEQVRPFKKEADYSYGMKE-----VCGDSFVLIGDAARFVDPIFSSGVSVALNSARIASGDIIEAVKNNDFSKSSFTHYEGMIRNGIKNWYEFITLYYRLNILFTAFVQDPRYRLDILQLLQGDVYLEVLDKMREIIAAVESDPEHLWHKYLG

General information: TITO was launched using: RESULT: Template: 5DBJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2398 -332147 -138.51 -814.08 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain D : 0.65 3D Compatibility (PKB) : -138.51 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.257

(partial model without unconserved sides chains): PDB file : Tito_5DBJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DBJ-query.scw PDB file : Tito_Scwrl_5DBJ.pdb: