Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------MDFEIRFLSFYVIQVE-GKDEQANKQFKHFQTLDTGE-FEESELKDFLDG------------------ELKKIVKRKADR-----HPQSEQVPTKIGHFIVEPGHELDSNPNYNMFNRARLAETKEDFNELSEQFVRTYLDTSAVRGGVFLVASAVPRKYFDESFVFIMKCDFEPKVARISDASSLIKKVEMA------------------ITTKNMKSIQY-PYMPEEGMVEEGELKIHQASHARYFEDFLKF-----VEYGES-MPEIMKNQV-MNMVQEHVYE-TFEDNSEELKQFEHDIE------------------------IWEASEKREIQERLDTHQVIEASAQIIEHTPEAQLKMKVGETEIKGLLADFGDSIHLAKVNGRYVALIEAETISFEKGSSPVEFYKPEGLHEVIERIRNKTEQD 4ZH0 Chain:A ((27-527)) QDLSDRYESLNNLLNRYSTLNTLIKLSADPSAINAVRENLGASAKNLIGDKANSPAYQAVLLAINAAVGFWNVVGYVTQCGGNANGQKSISSKTIFNNEPGYRSTSITCSLNGHSPGYYGPMSIENFKKLNEAYQILQTALKRGLPALKENNGKVNVTYTYTCSGDGNNNCSSQVTGVNNQK--DGTKTKIQTIDGKSVTTTISSKVVDSRADGNTTGVSYTEITNKLEGVP----DSAQALLAQASTLINTINNACPYFHAPKFSTTTGKICGAFSEEISAIQKMITDAQELVNQTSVINEHEQTTPVGNNNGKPFNPFTDASFAQGMLANASAQAKMLNLAEQVGQAINPERLSGTFQNFVKGFLATCNNPSQGSAPGTVTTQTFASGCAYVGQTITNLKNSIAHFGTQEQQIQQAENIADTLVNFKSRYSELGNTYNSITTALSNIPNAQSLQNAVSKKNNPYSPQGIDTNYYLNQNSYNQIQTINQEL---

General information: TITO was launched using: RESULT: Template: 4ZH0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1280 -3619 -2.83 -10.43 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -2.83 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.143

(partial model without unconserved sides chains): PDB file : Tito_4ZH0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZH0-query.scw PDB file : Tito_Scwrl_4ZH0.pdb: