Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKTKLTGRIVTRD--DPDYNEA--RTNINLSL----ERYPDIIVFCQNKQDALNALKWARENRVPFRIRGGRHSYENFSLLN----NGLVIDLSEMKKITVNQDKKLAYIEAGAELGEVYRTLWQY--GLTLPAGTIANVGLTGLTLGGGIGLLTRAAGLTCDSLVQLEMIVADEKEGADLITVSCSNHPDLFWASQGGGGGNFGIVTSMTFKAVPISQ-VSIFSITWGW-DDFEEVYNTWQNWAPYTDDRLTSSIEFWPK----EVNRIEALGQFVGPKTELKKLLKPLL-KAGSPTSGMVKTTPFIEAVTFFNS------PG----GN--QPQKMKRSGSFIEKPLSERAISTIKHFLEHAPNQNASVWQQALGGAAGRVAPDQTAFYYRDA-IIAQEYLTNWTSPGE-KRQNVRWIEGLRTSLSKE----TMGDYVNWPDIEI---------RNWPRTYYGENVERLRRVKTTYDPENVFRFEQSIPPLRRSLFF 4UD8 Chain:A ((52-530)) ----------FSPDQNATLFKEELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQ----ILDRAA-MGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGL-TKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEE--DSPLTIWNPYGGMMSRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEGETDAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTK------

General information: TITO was launched using: RESULT: Template: 4UD8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2593 -190204 -73.35 -445.44 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -73.35 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_4UD8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UD8-query.scw PDB file : Tito_Scwrl_4UD8.pdb: