Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATGKEKNAKNPMSLLIVLMAGLFLAILNQTLLNVAM-PHLMTEFGVSATTIQWLTTGYMLVNGVLIPLSAFLITRF-GQRSLFLVAMFCFTLGTLVCGIAP-NFSTMLIGRLIQAVGGGILQPLVMTTILLIFPPE-SRGKGMGIFGLAMMFAPAVGPTLSGWIIEH--------YTWRIMFYGLVPIGAIVIIVAFFIFKNMVEPQKIKLDTLGAILSIV-------GFASLLYGVSEAGSDGWTDPIVLSTVIIGAIAIVAFVVQQLRHDDPMLDFRVFKYDIFSLSSVINIIITVALYTGMFLLPIYLQNLVGFT---------ALQSGLLLLPGAIVMLIMSPISGILFDKFGP-RPLAIIGLLVTVVTTYQYTQLTI-----DTPYTHIMLIYSIRAFGMSLLMMPVMTAGMNQLPARLNSHGTAMSNTLRQISGSIGTSLITTIYTNRTTFHYSQIADKTSTADPNFLHAFQNAVSNLMVNMNVSYDTAKTYVYSHIYKHASLDSNVMGINDAFMWATLFCVAGLILSIFLRDVRKDKLRKKKKEELSLLPAPKEAKES 4TPH Chain:A ((21-449)) ----------------------ELWERFGYYGMQALIVYFMVQRLGFDDSRANLVWSACAALIYVSPAIGGWVGDKILGTKRTMLLGAGILSVGYALMTVPTENTWFMFSALGVIVVGNGLFKPNAGNLVRKIYE--SKIDSAFTIYYMAVNVGSTFSMLLTPWIKDYVNAQYGNEFGWHAAFAVCCVGILVGLGNYA-LMHKSLANYGSEPDTRPVNKKSLAIVLALAALSVVASAIIL-EYEDVARV---FVYAAGVAVLGIFFH-L-------ERAGLI---AALILTVQTVFFFIFYQQMSTSLALFALRNVDWDFQVFGTHLWTWSPAQFQALNPIWIMVLSPVLAW-------IAAKFALGFAVVAIGFFIYGFAGQFAVNGKTSSWVMIWGYASYSLGELLVSGLGLAMIAR---------MMGAYFVASGISQYLGGVVANFASV-----------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4TPH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1788 -278087 -155.53 -766.08 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -155.53 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.204

(partial model without unconserved sides chains): PDB file : Tito_4TPH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TPH-query.scw PDB file : Tito_Scwrl_4TPH.pdb: