Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDFSLELPVRTNKPRETGQSILIDNGYPLQFFKDAIAGASDYIDFVKFGWGTSLLTKDLEEKISTLKEHDITFFFGGTLFEKYVSQKKVNEFHRYCTYFGCEYIEISNGT---LPMTNKEKAAYIADFSD----EFLVLSEVGSKDAELASRQSSEEWLEYIVEDMEAGAEKVITEA-------------------RESG-TGGICSSSG----DVRFQIVDDIISSDIDINRLIFEAPNKTLQQGFIQKIGPNVNLANIPFHDAIALETLRLGLRSDTFFL 3FLU Chain:A ((28-206)) ---------------------------------------------------------------------------------------EQLRDLIDWHIENGTDGIVAVGTTGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTG--------ANNTVEAIALSQAAEKAGADYTLSVVPYYNKPSQEGIYQHFKTIAEATSIPMIIYNVPGRTVVSMTNDTILRLAE-IPNIVGVKEASGNIGSNIELINRAPEGFVV--LSGDDHTALPFMLCGGH------

General information: TITO was launched using: RESULT: Template: 3FLU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 658 -61311 -93.18 -414.26 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -93.18 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_3FLU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FLU-query.scw PDB file : Tito_Scwrl_3FLU.pdb: