Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEVKTISVEDTYEIRHRILRPHQSIEQCKYKEDHAEGSFHLGVFYDGTLISIASFSPQSQP-LLTEASAYRLRGMATLEGYRDQKAGSTLIKHAEHKLAESGVQAVWCNA---RHHVKGYYAKLGWKELGEPFDI-PGIG-NHIVMYKTLRTSR 1TIQ Chain:A ((60-171)) ---------------------------------------QFFFIYFDHEIAGYVKVNIDDAQSEEMGAESLEIERIYIKNSFQKHGLGKHLLNKAIEIALERNKKNIWLGVWEKNENAIAFYKKMGFVQTGAHSFYMGDEEQTDLIMAKTL----

General information: TITO was launched using: RESULT: Template: 1TIQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 444 -65529 -147.59 -618.19 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -147.59 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_1TIQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TIQ-query.scw PDB file : Tito_Scwrl_1TIQ.pdb: