Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAVIAKNEEQLKDAFYVREEVFVKEQN-VPAEEE-I-DE-LENESEHIVVYDGEKPVGAGRWRMK----------------DGYGKLERICVLKSHRSAGVGGIIMKALEKAAAD-GGASGFILNAQTQAVPFYKKHGYRVLSEKE-FLDAGIPHLQMMKD 1CJW Chain:A ((8-164)) ---FRCLTPEDAAGVFEIEREAFISVSGNCPLNLDEVQHFLTLCPELSLGWFVEGRLVAFIIGSLWDEERLTQESLALHRPRGHSAHLHALAVHRSFRQQGKGSVLLWRYLHHVGAQPAVRRAVLMCEDALVPFYQRFGFHPA-GPCAIVVGSLTFTEMHC-

General information: TITO was launched using: RESULT: Template: 1CJW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 606 -95614 -157.78 -708.25 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -157.78 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.402

(partial model without unconserved sides chains): PDB file : Tito_1CJW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CJW-query.scw PDB file : Tito_Scwrl_1CJW.pdb: