Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPLHENLAGKTAVITGGSGVLCSAMARELARHGMKVAILNRTAEKGQAVVKEITAAGGTACAVAADVLDRMSLERAKEDILGQFGAVDLLINGAGGNHPDAITDVETYEEAGEGQSFFDMDERGFLTVFSTNFTGAFLASQVFGKELLKADSPAIINLSSMSAYSPMTKVPAYSAAKASINNFTMWMAVHFAETGLRVNAIAPGFFLTKQNHDLLINQDGTFTSRSHKIIAGTPMKRFGKPEDLLGTLLWLADESYSGFVTGITVPVDGGFMAYSGV 4IBO Chain:C ((23-269)) ------DLGGRTALVTGSSRGLGRAMAEGLAVAGARILINGTDPSRVAQTVQEFRNVGHDAEAVAFDVTSESEIIEAFARLDEQGIDVDILVNNAGIQFRKPMIELETAD---------------WQRVIDTNLTSAFMIGREAAKRMIPRGYGKIVNIGSLTSELARATVAPYTVAKGGIKMLTRAMAAEWAQYGIQANAIGPGYMLTDMNQALIDNPE--FDA---WVKARTPAKRWGKPQELVGTAVFLS-ASASDYVNGQIIYVDGGMLS----

General information: TITO was launched using: RESULT: Template: 4IBO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1337 -131714 -98.51 -533.25 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -98.51 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_4IBO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IBO-query.scw PDB file : Tito_Scwrl_4IBO.pdb: