Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTIKDIAKLANVSHTTVSRALNNSPYIKEHTKKKILELAEQLNYTPNVNAKSLAMQKSHTIGLFFTSITNGTSHSFFADTIKGVNQ-AISEDYNLYVRGID-----DLKNYDSVTPMRYDGIILMSQSDIDNSFIYHIREKNIPLVVLNRDIDDRTITNILSNDKEGS-QEAVEYFIQSGHQDIAIIEGIEGFKSSQQRK-EGYLSALIQHHIPIKHEYSVKGQYDMESGFQAMERLLALPNPPTAVFCSNDDMAIGAMNAIFAKGLRVPDDISVIGFDDIGFSQYITPRLSTVKRPVEKISVLGAQKLLSLISEPETKAEKILENTEFMVRDSVRRLTT 3OQN Chain:C ((3-320)) -ITIYDVAREANVSMATVSRVVNGNPNVKPTTRKKVLEAIERLGYRPNAVARGLASKKTTTVGVIIPDI----SSIFYSELARGIEDIATMYKYNIILSNSDQNMEKELHLLNTMLGKQVDGIVFMG-GNITDEHVAEFKRSPVP-IVLAASVEEQEETPSVAIDYEQAIYDAVKLLVDKGHTDIAFVSGPMAEPINRSKKLQGYKRALEEANLPFNEQFVAEGDYTYDSGLEALQHLMSLDKKPTAILSATDEMALGIIHAAQDQGLSIPEDLDIIGFDNTRLSLMVRPQLSTVVQPTYDIGAV-AMRLLTKLMNKEPVEEHIVE---------------

General information: TITO was launched using: RESULT: Template: 3OQN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1645 -170174 -103.45 -548.95 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -103.45 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_3OQN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OQN-query.scw PDB file : Tito_Scwrl_3OQN.pdb: