Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAQKTFKVTADSGIHARPATVLVQTASKYDADVNLEYNGKTVNLKSIMGVMSLGIAKGAEITISASGADENDALNALEETMKSEGLGE 1Y4Y Chain:A ((2-88)) -AEKTFKVVSDSGIHARPATILVQTASKFNSEIQLEYNGKTVNLKSIMGVMSLGIPKGATIKITAEGADAAEAMAALTDTLAKEGLAE

General information: TITO was launched using: RESULT: Template: 1Y4Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 413 -51919 -125.71 -596.76 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.93 3D Compatibility (PKB) : -125.71 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.744

(partial model without unconserved sides chains): PDB file : Tito_1Y4Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y4Y-query.scw PDB file : Tito_Scwrl_1Y4Y.pdb: