Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYTVTLNPSVDYIVHVEDFTVGGLNRSSYDTKYPGGKGINVSRLLKRHHVASKALGFVGGFTGEYIKTFLREENLETAFSEVKGDTRINVKL---KTGDETEINGQGPTISDEDFKAFLEQFQSLQEGDIVVLAGSIPSSLPHDTYEKIAEACKQQNARVVLDISGEALLKATEMKPF-LMKPNHHELGEMFGTAITSVEEAVPYGKKLVEQG-AEHVIVSMAGDGALLFTNEAVYFANVPKGKLVNSVGAGDSVVAGFLAGISKQLPLEEAFRLGVTSGSATAFSEELGTEEFVQQLLPEVKVTRL 3N1C Chain:C ((4-290)) -IYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQGIRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFGVAAGSAATLNQ--------------------

General information: TITO was launched using: RESULT: Template: 3N1C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1598 -153257 -95.91 -543.46 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -95.91 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_3N1C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N1C-query.scw PDB file : Tito_Scwrl_3N1C.pdb: