Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNTMKRMFCSMTVLVTAPYNEEGRKELENLFGSVAYQSWKEQGRAYREDELIQLLKATNATGLITELDQVTDS-VFASVPELSFVGVCRGMPSNVDVAAASKRGIPVFYTPGRNAQAVAEMFIGNVISFLRHTSASNQWLKDGEWDSDYLQAYVK-FKGNELTGKTVGMIGFGAVGQRIAKLLTAFDCKIKYYDPYIQDDHPLYEKASLKTV---FSDSDIVSVHLPRTEETLGLIDRQYFDLMKESAIFVNTSRAVVVNREDLLFVLKEHKISGAILDVFYHEPPEESDYELISLPNVLATPHLAGATFEVEDHHVTILNKALKKWK-GEKTLNIQTMYNKDALKTGG 2DBR Chain:F ((5-327)) -------------VFITREIPEVGIKMLEDEF---EVEVWGDEKEIPRE---ILLKKVKEVDALVTMLSERIDKEVFENAPKLRIVANYAVGYDNIDIEEATKRGIYVTNTPDVLTDATADLAFALLLATARHVVKGDRFVRSGEWKKRGVAWHPKWFLGYDVYGKTIGIIGLGRIGQAIAKRAKGFNMRILYYSRTRKEEVERELNAEFKPLEDLLRESDFVVLAVPLTRETYHLINEERLKLMKKTAILINIARGKVVDTNALVKALKEGWIAGAGLDVFEEEP--YYNEELFKLDNVVLTPHIGSASFGAREGMAELVAKNLIAFKRGEIP---PTLVNREVIK---

General information: TITO was launched using: RESULT: Template: 2DBR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1671 -24742 -14.81 -78.05 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain F : 0.74 3D Compatibility (PKB) : -14.81 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_2DBR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DBR-query.scw PDB file : Tito_Scwrl_2DBR.pdb: