Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKAIQSSMWREFGLMCTVGIGDNMLLSKLALDLESKKTKSGIARWRYEDVPNRLWKVR-PLSKMWGIGGRMERNLNRMGISTVGQLAKFPLELLEKKFGI-MGNQLYYHAHGIDLSEIGAPLMQGQISFGKSQILFRDYTKREEIKAVLLEICEEVARRARTYNKVGRTISLGIGYSKDE-LGGGFHRSKTIDFP----TNIT---MDIYKCCLMFFNKFYSGKKQ 3OSN Chain:A ((179-396)) IAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPN---QQTVLLPESCQHLI-HSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGISVAQRIQKLSFGEDNSPVILS--GPPQSFSEEDSFKK-CSSEVEAKNKIEELLASLLNRVCQDGRKPHTVRLIIRRYSSEKHYGRESRQCPIPSHVIQK---YDVMTPMVDILMKLFRNMVN----

General information: TITO was launched using: RESULT: Template: 3OSN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 613 12109 19.75 59.65 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 19.75 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_3OSN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OSN-query.scw PDB file : Tito_Scwrl_3OSN.pdb: